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KMID : 0043320080310101339
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Archives of Pharmacal Research
2008 Volume.31 No. 10 p.1339 ~ p.1345
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Production, Isolation and Biological Activity of Nargenicin from Nocardia sp. CS682
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Yoo Jin-Cheol
Sohng Jae-Kyung
Simkhada Jaya Ram
Park Seong-Chan
Yamaguchi Tokutaro
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Abstract
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Culture broth of an actinomycete isolate, Nocardia sp. CS682 showed specifically higher antibacterial activity against methicilin resistant Staphylococcus aureus (MRSA). Purified substance from the organism, CS-682, which is active against MRSA and Micrococcus leuteus, is a C28H37NO8 (M+H+, observed: 516.83) and identified as an unusual macrolide antibiotic, nargenicin. The chemical structure of CS-682 was identified by FT-IR, 1H-NMR, 13C-NMR, and (1H-1H and 1H-13H) COSY. The anti-MRSA activity of CS-682 was stronger than that of oxacillin, vancomycin, monensin, erythromycin, and spiramycin. Phylogenetic analysis showed that strain CS682 is closely related to Nocardia tenerifensis DSM 44704T (98.7% sequence similarity), followed by N. brasiliensis ATCC 19296T (98.4% sequence similarity). The ability of Nocardia sp. CS682 to produce nargenicin was unique.
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KEYWORD
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MRSA, Nargenicin, Nocardia sp. CS682, Antibiotic, Structure study, Biological activity
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